Genetic Transformation Effects Of Introducing The Gfp...

Valerie Sword Guadelupe Aguirre Life 102 L 17 2 December 2014 Genetic Transformation: Effects of Introducing the GFP Gene into E. Coli Bacteria Introduction: When a bacterial cell incorporates foreign DNA into its own genome, this is known as bacterial transformation, a form of genetic transformation. There are several ways to transform genetic material; projectile bombardment, electroporation, and heat shock. This experiment uses the heat shock method. Interest in genetic transformation began as early as 1928 when Frederick Griffith performed an experiment with pneumococcus bacteria and mice, which suggested the possibility of bacterial transformation (Griffith 1928). Expounding upon this discovery, other experiments proved that DNA was†¦show more content†¦This experiment demonstrates genetic transformation and the way genes are expressed, which is important for a multitude of reasons. Gene transformation can be used in many ways, including but not limited to: crop modification, medical research, and bioremediation. Materials and Methods: For this lab we used two microcentrifuge tubes, a pair of vinyl gloves to prevent contamination, a micropipetter, six sterile micropipetter tips, 500  µl of a transformation solution containing CaCl2, seven sterile loops, ice, a timer, a 42 °C water bath, a floating rack two colonies of E. Coli bacteria, pGLO plasmid DNA, four Luria Bertani (LB) broth nutrient agar plates (one with LB alone, two with LB and ampicillin, and one with LB, ampicillin, and arabinose), 500  µl of LB nutrient broth, tape, a UV light, and a 37 °C incubator. We labeled the two microcentrifuge tubes, one â€Å"+pGLO† and the other â€Å"-pGLO†, then put on our gloves. We pipetted the 500  µl of transformation solution into these two tubes, half (250  µl) in each, and put these tubes on ice. Then we put a colony of E. Coli bacteria into each tube and put pGLO plasmid DNA into the +pGLO tube, using sterile loops. We put the tubes back on ice, timing it for 10 minu tes. During the interim, we retrieved and labeled the bottom of each of the four LB nutrient agar plates, according to what they contained and would contain. We then took both tubes out of the ice and immediately placed them into a floating rack

Implementation Of Sql Server Database And Business...

IMPLEMENTATION OF SQL SERVER DATABASE AND BUSINESS INTELLEGINCE IN XXXXX By Pranay Papishetty Bachelor of Technology (IT), Jawaharlal Nehru Technological University, India 2011 A Capstone Project Submitted to the Graduate Faculty Of St. Cloud State University In Partial Fulfillment of the Requirements For the Degree Master of Engineering Management St. Cloud/St. Paul, Minnesota [November, 2014] ACKNOWLEDGEMENTS Foremost, I would like to convey my honest gratitude to my mentors Dr. Hiral Shah and Dr. Ben Baliga for their continuous and tremendous support of my Master’s study at St. Cloud State University. I’m really thankful for all the knowledge they shared and guiding me towards the right path of success. Special thanks to Prof. Gary for spending his valuable time in reviewing this report and for all his insightful suggestions and recommendations. Finally I would like to thank my parents and friends for their support and encouragement. ABSTRACT The main objective of this project was to meet the needs of data management, capturing data from various data sources and integrating the data into a database for reporting purposes. This was achieved previously using a legacy system called as FOCUS and is now replaced by a database using SQL Server Business Intelligence. SQL Service Business Intelligence is not only used to load data into the database but also provides tools for reporting and analysis services. Loading data is done using a tool provided by SQL Server called

Romanticism and Neoclassical Free Essays

ROMANTICISM AND NEO-CLASSICAL Romanticism a word that makes one think that it is a piece of art that shows love, a man and a woman. But it is not quite that, romanticism can mean freedom, rebellion, it could symbol intuition, emotion, the individual, and truth. It refers to art work that states feelings, moods, and dominates. We will write a custom essay sample on Romanticism and Neoclassical or any similar topic only for you Order Now An individual expression of experiences which cannot and could not be evaluated or assessed in purely rational or materialistic terms. Romanticism was one of the most unique ism that would most certainly be remembered most. Romanticism started during the time of Neo-Classicism, many disliked the view that Neo-Classicism and so they began a new style. Romanticism valued human emotions, instincts, over rational, rule based approach to questions of value and meaning in the arts, society, and politics. Romanticism can be charactized by formal stylization; the compositional is simplification, and a preference for graphic techniques and expanses of color. Another thing that also inspired the art movement was the attitude towards the landscape. However romanticism wasn’t accepted until 1830. The intention for Romanticism was to create a new world to enter the wreckage of the old; the time for innovation, experiment, new social systems and Utopias, new concepts and morality. A romantic was one who had broken loose from the rigid controls of the past and felt free to move ahead. Romantic artists explored specific values of individuality which Neo-Classicism ignored; the values of intuition, instinct, and even the more in accessible aspects of feelings which reach and exceed the boundaries beyond of reason. There were four non art history facts that were either influenced or affected the art movement were; the American and French Revolutions, the restoration between the Greeks and Turks, and the Age of the Enlightenment. The Enlightenment had a negative effect on the romantics; they attacked the Church. The two artists that are quite interesting to learn about from this period are Eugene Delacroix and Theodore Gericault. They might not be Michelangelo or Paul Klee or any other big shot artist but they are still artists, artists that have done beautiful work and some most incredible art pieces. Eugene Delacroix, born on April 26, 1791, in the month of the Taurus, in Paris suburb called Charenton-Saint-Maurice. He was presumed to be premature, but some expect that his real father was Charles Maurice de Talleyrand, instead of Charles-Francois Delacroix. However Delacroix turned a blind ear to them for he believed that Charles-Francois was his true father. He showed an exceptional talent for music, for the cathedral; who had been a friend of Mozart, Delacroix learnt how to play the piano, violin, and the guitar. He was only nine or ten when according to his friend critic Theophite Silvestre, when he went to Louvre. When he was seven his father died, his mother packed up everything and took Delacroix and Henriette and left to live in Paris. His two older brothers were away at war. He was taught by Pierre-Narcisse Guerin and also by Theodore Gericault at Lycee Louis-le-Grand. However he was not on good terms with Theodore; who was seven years his senior to Eugene. Eugene had turned a deaf ear to Gericault’s injunction from the first time he meet him. He felt an instinctive affinity to Theodore’s ideas. It wasn’t until 10 years later after they met that Gericault died at age 32. His art piece Bark of Dante was debt to Theodore Gericault; who he met. Everywhere in his art one can see in the exploitation of the dramatic potential in the waterscape, or in the use of diagonals to convey the sense of struggle and movement in the form of the figures. The bold emphasis on their musculature is incredible. However the theme is and was a thoroughly respectable one. It was free of anything that might rile official dom. After it had been exhibited at the Salon, the French government paid 2,000 francs for it. In his later years he became called â€Å"a volcanic crater artistically concealed behind bouquets of flowers† or even sometimes called The Great Romantic. He could be a lover of women and a work fanatic, an adept at social trivia and a man of wider ranging erudition not only mastery of esthetics but an impressive grasp of music, theater, and literature. His first foreign journey was to England, where he learnt how to ride on horses, which would come in handy for the Moroccan desert. Where he went mostly for politic reasons and not only was it for art it was also to escape the civilization of Paris. He produced over 100 sketches and paintings of the people, their costumes or just the landscape. He demonically turned out more than 850 paintings, thousands of sketches, watercolors, and drawings of art. In his lifetime he produced more than 20 works that were inspired by Shakespeare. He continued to make art till he died; for he was trying to reconcile opposites to see art as a whole. For part of Eugene’s genius laid in his capacity to learn from others. He died in 1863 in Paris, France. One of his artwork titled Orphan Girl at Cemetery which was worked and finished between 1823-1824. Delacroix used oil on canvas with this art. It shows a girl with hair pilled on her head and she is looking to sky. In the background you can just see the church and some crosses. There is a sense of sadness and loneliness in her eyes and her look. Theodore Gericault was born in 1791 into a bourgeois family in Rouen. Gericault moved to Paris as a boy. He has been fascinated by all aspects of equestrian such as races, jumping and riding schools. He was also overwhelmingly attracted by the clashes between individuals; he investigated their various forms in journeys which in England led him to observe the human deluxe. Theodore was educated in the tradition of English sporting art by the Carle Vernet, and even by Pierre-Narcisse Guerin, who disliked his temperament but saw a talent in him. He then left and learnt at the Louvre for six years when he realized that he preferred the vitality over the prevailing school of Neo-Classicism. He exhibited his Wounded Cuirassier at the Salon in 1814 and also his first major work The Charging Chasseur at the Salon in 1812. Gericault was a merry, gregarious man whose tastes as a bon vivant did not preclude a deep-seated sympathy for the under dog. He went to Florence, Rome, and Naples in 1816-1817, mostly to escape a romantic entanglement with his aunt. Gericault became fascinated by Michelangelo; which helped inspire his art piece the Race of the Barberi Horses. After he went back to France in 1821 he painted a series of portraits of his friend Dr. Etienne-Jean Georget’s patients; each containing a different diagnosis. Theodore drew his subjects from the crudest parts of reality; he visited slaughter houses, morgues, asylums, delving into the morbid events reported in newspapers, observing the devastating corporeal strength of animals. Some of his artworks consist of horses, lions, and tigers. Gericault was also one of the first artist to take up the newly invented process of lithography, producing a serveing of 13 pickes illustrating the life of the English poor. He was in the process of painting new artworks, when his health stroked a final note. Theodore was always riding for his among his passions was horses. He owned them, painted them, and even tamed them. His fatal illness grew on to a riding trip which injured his spine and caused him to waste. He died after a slow period of suffering, in Paris 1834 at the age of 3 Art History: Neoclassicism: (1750 – 1830) The term Neoclassicism refers to the classical revival in European art, architecture, and interior design that lasted from the mid-eighteenth to the early nineteenth century. This period gave rebirth to the art of ancient Rome and Greece and the Renaissance as an opposition to the ostentatious Baroque and Rococo art that preceded the movement. Although the movement spread throughout Western Europe, France and England were the countries that used the style most frequently in their arts and architecture, using the classical elements to express ideas of nationalism, courage, and sacrifice. The movement was inspired by the discovery of ancient Italian artifacts at the ruins of Herculaneum and Pompeii. Also influential in the development was the cultural studies of German art historian Johann J. Winckelmann who claimed that the most important elements of classical art were â€Å"noble simplicity and calm grandeur. † Neoclassicism emphasized rationality and the resurgence of tradition. Neoclassical artists incorporated classical styles and subjects, including columns, pediments, friezes, and other ornamental schemes in their work. They were inspired by the work of Homer and Plutarch and John Flaxmann’s illustrations for the Illiad and Odyssey. Other classic models included Virgil, Raphael, and Poussin among others. Neoclassical painters took extra care to depict the costumes, settings, and details of classical subject matter with as much accuracy as possible. Much of the subject matter was derived from classical history and mythology. The movement emphasized line quality over color, light, and atmosphere. The height of Neoclassicism was displayed in the paintings of Jacques-Louis David and Jean Auguste Dominique Ingres. How to cite Romanticism and Neoclassical, Papers

Business Environmental Analysis Samples †MyAssignmenthelp.com

Question: Discuss about the Business Environmental Analysis. Answer: Introduction Technology plays a great role in conducting the day to day operations of the business. Activities of the organization are conducted electronically because of the internet access in every company. Computers are present in every organization to complete the specific tasks. Technology at the workplace has brought a drastic change to complete the work. The technology like computers removed the workplace boundaries and also helps to take correct decisions at your workplace. So, in this report the emphasis will be given on the use of computer in the modern workplace. At the modern workplace technology is used so that the task can be completed fast and the organization can easily attain goals and objectives. Modern workplace The computer system is used to simplify the work and also to complete the work on time. Latest technology should be used by every organization, so that it can be easy for the organization to attain the goals and objectives. A computer gives knowledge to the workers about the tasks and operations that should be performed in a proper way. Computer is an important technology that should be taken into consideration by the organization to attain the success in the market (Raspotnig and Opdahl, 2013). Without the use of computer or laptop it is very difficult for an organization to maintain the records. Computer is very essential to record the transactions and also to keep a proper record of the employees who are conducting the activities in the business. Computer is defined as an electronic machine that helps to store, organize and search the information. The important components of computer are hardware and software. The computer system has various modern components like primary storage, input devices, output devices and communication devices (Arachchilage and Love, 2014). Hardware and Software components The hardware components of the computer system are: CPU: It is considered as a hardware component of the computer system in which the manipulation of the symbols are done. The full form of CPU is Central processing unit. It stores the data and the instructions of programs temporarily before or after processing. It is considered as a brain of the computer. CPU is considered as an electronic circuit that carries the instructions which are given by the computer. CPU executes instructions which are given by a computer program and also it take into consideration basic arithmetic and logical operations. It is analyzed that the speed and efficiency of the computer is dependent on the CPU. The basic function of CPU is that it controls all the parts of machines and sends time signals. It helps to transfer the data between the various devices and decodes the instructions. Also it executes programs stored in the memory and performs communication among the Devices (Gartshore and Blake, 2014). Computer Keyboard: It contains many mechanical switches which are called keys. The keyboard can be wireless and also connected with wires. Wireless keyboard are connected via Bluetooth. It is an input device which helps to enter the letters, numbers and also the symbols into a computer. It is known as one of the common technique which is used for writing. Mouse: It is considered as a pointing device that has two dimensional motions that are related with the surface. It helps to control the graphical user interface. Mouse is designed in such a manner in which two buttons and a scroll wheel is seen (Grkaynak, Yilmaz and Taskiran, 2014). The software components of the computer system are: Operating system software: It helps to load all the programs at the time when the computer starts. It uses the characteristics of a computer without having the knowledge of the hardware information. The operating system helps the computer to have the knowledge of bits and bytes. All the functions of a computer system can be conducted by using the operating system software. Without this software it will be difficult to operate the activities in an effective manner (Lee, Connor and Bourgoin, 2014). Application software: This software can be used when the operating system is loaded in a proper way. It contains many programs like word processing programs, spreadsheets and databases. Microsoft word is considered as a part of word processing applications (Larson, Menagarishvili, Rubinoff, Smith and Waters, 2016). Advantages and threats of computer in the workplace There are various benefits and advantages that are connected with the computer. Computer helps to record the transactions in an accurate manner. Computer is a technology that simplifies the work of an individual. It reduces the workload of the employees or the workers who are working in the company. There are various software that are used with the help of the computer, in which the proper record of the files, documents are kept. The information of the businesses is organized in a very proper manner (Galegher, Kraut and Egido, 2014). The large amount of data can be stored in a convenient and inexpensive way. The computer has the ability to organize the organization files in an efficient manner, so that there can be enhancement in the time management and also in the level of productivity. For example: It is very easy for the company to make the training material. The operations of the business can be completed in a speedy way and also it help to collect the consumer feedback, ordering the raw material. It helps the organization to save time and enhances the better quality outcomes. The high sales and profits can be generated by using the company website. There are many businesses that conduct their operations online and allow the customers to shop for their products. These all activities can be taken into consideration by using the latest technologies like computers (De Cocker, De Bourdeaudhuij, Cardon and Vandelanotte, 2016). The main threat that is emerged by the use of computers in the workplace is concerned with the loss of confidential information. It is seen that confidential information are leaked from the computer. The company or the manager saves information in the computer and when it is leaked it is a threat for the organization. Loss of information can also reduce the profitability of the organization (Nelson,Phillips and Steuart, 2014). Physical Danger Ergonomics is considered as a scientific study of a person and also their relation with the working environment. The individual in the organization suffers pain at the time of conducting the operations of the organization. There are many factors that are concerned with the ergonomically related problems. The person who sits for a long time in front of computer faces various different problems. If an individual do not work or conduct its operations with a good posture, then it is very difficult to survive in an organization. Good posture helps the individual to work in effective way. If the person or an individual gives excessive force on the body parts, then it can result in excessive pain (Eijckelhof, et al., 2013). For example: Poor posture can be determined if the keyboard and the monitor is not kept in a straight line from the seat. The awkward position at the workplace can lead to severe stress in the neck, wrist and shoulders. So, to conduct the operations or the activity it is very essential for an individual to carry a good posture. It is seen that if an individual conducts its operation in a same position for a long time then it can enhance the possibility of injury. The organization should give comfortable seats, so that the employees can sit in a good posture and can conduct their task effectively (Chory, Vela and Avtgis, 2016). It is very essential for an individual to have a proper knowledge of the positions that can minimize the risk of injury. It is seen that there are many individuals who faces the severe problem of neck pain. It is due to the wrong position in which the individual is working. It is very important for an individual to be aware about the risk factors that can result in musculoskeletal problems. If an individual is aware then it can be easy to avoid the problem at the time of using computer (Hoepman, 2014). Data protection legislation Data protection legislation requires every organization to take into consideration the personal information so that the essential principles concerned with privacy and security can be implemented. It is very essential to make sure that the information is accurate and can be handled according to data protection rights of an individual. It is the duty of information commissioner officer to take into consideration the rights that assures about the information of an individual. The principles that are related with privacy and disclosure should be used to handle the personal information of an individual. Conclusion By taking into consideration this report, it is concluded that computers are the essential technology that should be adopted by every organization. It is very beneficial and also helps the organization to maintain the records in efficient way. In the recent scenario, it is analyzed that without computer or laptop not even a small business can survive. Computer is an essential requirement for every organization to sustain in the competitive environment. References Arachchilage, N.A.G. and Love, S., 2014. Security awareness of computer users: A phishing threat avoidance perspective.Computers in Human Behavior,38, pp.304-312. Chory, R.M., Vela, L.E. and Avtgis, T.A., 2016. Organizational surveillance of computer-mediated workplace communication: Employee privacy concerns and responses.Employee Responsibilities and Rights Journal,28(1), pp.23-43. De Cocker, K., De Bourdeaudhuij, I., Cardon, G. and Vandelanotte, C., 2016. The effectiveness of a web-based computer-tailored intervention on workplace sitting: a randomized controlled trial.Journal of medical Internet research,18(5). Eijckelhof, B.H.W., Huysmans, M.A., Garza, J.B., Blatter, B.M., Van Dien, J.H., Dennerlein, J.T. and Van Der Beek, A.J., 2013. The effects of workplace stressors on muscle activity in the neck-shoulder and forearm muscles during computer work: A systematic review and meta-analysis.European Journal of Applied Physiology,113(12), pp.2897-2912. Galegher, J., Kraut, R.E. and Egido, C., 2014.Intellectual teamwork: Social and technological foundations of cooperative work. Psychology Press. Gartshore, E. and Blake, H., 2014. Development and evaluation of a computer based e-learning tool to enhance knowledge of workplace wellness in a healthcare setting. Grkaynak, G., Yilmaz, I. and Taskiran, N.P., 2014. Protecting the communication: Data protection and security measures under telecommunications regulations in the digital age.Computer law security review,30(2), pp.179-189. Hoepman, J.H., 2014, June. Privacy design strategies. InIFIP International Information Security Conference(pp. 446-459). Springer, Berlin, Heidelberg. Larson, B., Menagarishvili, O., Rubinoff, A., Smith, J. and Waters, R., 2016. Technical Communication in the Workplace: Computer Science, Business, and Law. Lee, C., Connor, T. and Bourgoin, D., 2014. Human Factor Approach to Computer Documentation Design, Implemenatation, and Workplace Ergonomics.Journal of PeriAnesthesia Nursing,29(5), p.e12. Nelson, B., Phillips, A. and Steuart, C., 2014.Guide to computer forensics and investigations. Cengage Learning. Raspotnig, C. and Opdahl, A., 2013. Comparing risk identification techniques for safety and security requirements.Journal of Systems and Software,86(4), pp.1124-1151.